New ClickGene Publication in Nucleic Acids Research

The Kellett and Brown groups recently published a collaborative article "Di-copper metallodrugs promote NCI-60 chemotherapy via singlet oxygen and superoxide production with tandem TA/TA and AT/AT oligonucleotide discrimination" in the Oxford journal, Nucleic Acid Research. The work showed how di-nuclear metal complexes with artificial nuclease activity promote cancer cell death and selectively bind DNA sequences with TA/TA or AT/AT steps. The compounds were active against solid epithelial cancer cells from the National Cancer Institute’s 60 human cell line panel (NCI-60) indicating exciting future therapeutic applications for this class of molecule.

3rd Network Meeting – University of Oxford

PIs and ESRs were reunited from 19 - 21 March 2018 for the third Annual ClickGene Meeting in Oriel College at the University of Oxford (UOXF). Organised by the ClickGene partners UOXF and ATDBio, the annual meeting included workshops, industry and laboratory visits, keynote lectures and ESR presentations.

On the first day, Dr. Jeanette Müller from accelopment AG provided the ESRs with an expert training session on European Grant Application Writing. ESRs were informed about existing EU funding opportunities for young researchers and different steps related to proposal writing. In the afternoon, ESRs had the opportunity to visit the premises of the companies ATDBio Ltd. and Oxford Nanopore Technologies in the Oxford Science Park and they also had the opportunity to visit the research laboratories of Prof. Tom Brown in UOXF.

During the second day, ESRs, PIs and others attended nine cutting-edge lectures by ClickGene PIs and researchers from the UOXF as follows:

Tom Brown, Department of Chemistry, UOXF

“Clicking DNA”

Chrys Chatgilialoglu, CNR

“Our radical approach to biomimetic chemistry.”

Andrew Kellett, Dublin City University

“Molecular Methods for Probing Non-Covalent Metallodrug-DNA Interactions”

Rebecca Andrews, Department of Physics, UOXF

“A single-molecule sequencing method based on DNA binding”

Jonathan Doye, Department of Chemistry, UOXF

“Coarse-grained modelling of DNA”

Graham McClorey, Department of Physiology, Anatomy and Genetics, UOXF

“Cell-Penetrating peptides to enhance oligonucleotide delivery”

Andrew Turberfield, Department of Physics, UOXF

“Molecular machinery from DNA”

Jack Hardwick, Department of Chemistry, UOXF

“F-DNA: A new form of the DNA Double Helix”

Thomas Carell, LMU Munich

“DNA bases beyond Watson and Crick”

On the third and last day, each ESR presented recent advancements in their research projects with the other ESRs and the ClickGene PIs. The ClickGene coordinator Andrew Kellett held a presentation on the status of the ClickGene project, in its last months before completion. A board meeting reuniting PIs and ESR representatives put an end to this 3-day event.

The next network meeting will take place in Dublin, Ireland by the end of the year. Information will follow in due time.

ClickGene presented results at SCNAC 2017

5 ESRs presented their project and results during the 17th Symposium on Chemistry of Nucleic Acid Components in Český Krumlov, Czech Republic. The conference included 52 oral presentations and 97 poster presentations from 5 to 7 June 2017. It was hosted by one of the ClickGene partners, the Institute of Organic Chemistry and Biochemistry AS CR.

ESRs presented the following posters:


ClickGene PIs and ESRs attend Symposium on Chemistry of Nucleic Acid Components

Most of the ClickGene PIs and ESRs will be attending the 17th Symposium on Chemistry of Nucleic Acid Components (SCNAC) in Český Krumlov, Czech Republic, held in June of this year. Last year’s event attracted more than 150 participants from 21 countries. The programme consists of 10 plenary lectures, 4 "Emerging young investigator" invited lectures, ca. 40 short oral presentations and a poster session.

Supervisor Michal Hocek to chair the symposium

Michal Hocek, supervisor of two ESRs for ClickGene, is the chairman of the SCNAC symposium. Michal’s main research areas include the chemistry of nucleobases, nucleosides, nucleotides and nucleic acids and his ESRs are studying in the area of genome editing and gene knockout.

Invited speakers

The list of invited speakers includes Thomas Carell of the Ludwig-Maximilians-University Munich, Germany. Thomas is, like Michal, the supervisor of two ESRs for the ClickGene project. Additionally, he is the lead of WP1 Materials for Genome Editing. Other speakers include Cynthia Burrows from the University of Utah, Kurt Vesterager Gothelf from the Interdisciplinary Nanoscience Center, Aarhus University and Andreas Marx from the University of Konstanz. The full list of plenary invited speakers is available on the SNAC website.

How to attend

The registration for the event is open and available on the SCNAC website. Due to limited capacity of the venue, the registration will be restricted to ca. 150 regular participants.

baseclick GmbH launches a new and enhanced click chemistry-based kit for detecting cell proliferation

baseclick GmbH – one of the industry partners of ClickGene – is very excited to announce their latest innovation, developed within the frame of ClickGene: a new and significantly enhanced click-chemistry based EdU “DetectPro”-Kit for the analysis of cell proliferation.

The baseclick EdU Kits provide a superior alternative to BrdU and [3H] thymidine assays by incorporating the thymidine analogue EdU during DNA synthesis and then using click chemistry for detection in a variety of dye fluorescent readouts. No harsh, DNA denaturating and laborious antibody based detection is required.

The new DetectPro Kits combine all the advantages of their very popular EdU Click Kits with a brand new fluorescence enhancer system. This makes them as easy to handle and as reliable as their predecessor, while showing outstanding efficiency and greatly enhanced signal and sensitivity. With key features such as low cell number requirement paired with low signal-to-noise ratios, baseclick  is on the forefront to the ultimate goal of “low content diagnosis”.

The new DetectPro Kits will be offered for three different readout systems: cell proliferation analysis by imaging, by flow cytometry and by high throughput screening analysis (HTS). Please visit the baseclick GmbH website for more detailed information on their new click-chemistry based tool.


Typical EdU cell proliferation assay: Incubation of HeLa cells for 30 minutes with EdU followed by click reaction guided detection using fluorescence microscopy. While in baseclick's standard EdU cell proliferation assay at such short exposure times (here 15 ms) only show very light signals of proliferating cells (middle image), the newly invented EdU DetectPro assay (right image) is able to detect cell proliferation with even higher efficiency and sensitivity with minimal background.

New ClickGene publication in Angewandte Chemie

ClickGene ESR Ján Matyašovský is among the authors of a new publication in Angewandte Chemie (made available as open access): 

Matyašovský, J., Perlíková, P., Malnuit, V., Pohl, R., & Hocek, M. (2016). 2-Substituted dATP Derivatives as Building Blocks for Polymerase-Catalyzed Synthesis of DNA Modified in the Minor Groove. Angewandte Chemie, 128(51), 16088-16091.

ClickGene Mid-Term Meeting

The ClickGene Mid-Term Meeting will be held from the 29th November to the 1st December in Bologna Italy. The event will consist of three thoroughly planned days:

  • On the first day the programme network meeting will be held
  • On the second day the technical review meeting will take place
  • On the final day the ESRs will be treated to a quality management workshop presented by baseclick GmbH

The Programme Network Meeting will consist of an update of the project as a whole. The coordinator will give a short presentation on the status followed by the individual WP leaders. The next hours will be reserved for the ESRs. They will present their work progress to the consortium.

The ESRs will once again be busy during the Technical Review Meeting. The will each give a short presentation telling the consortium their background, their work and training experience in the network. They will also receive a short feedback on their presentations.

The location for the event will be the ISOF Institute and the EC Project officer and a technical expert will be present for the whole meeting.

This meeting follows the 2nd Annual Network Meeting that was held in Grenoble from the 25th to 27th of March.

Mid-Term Meeting this winter

Planning for the ClickGene Mid-Term Meeting is underway and progress is looking good. We will be hoping to avoid the freezing weather by holding it in Bologna, Italy from the 29th November to the 1st December. The participants are expected to arrive on the 28th November and the first day of work will start the next morning. On the first day the Programme Network Meeting will be held. This includes an update of the project status by the coordinator and updates on the individual Work Packages by their respective leaders. Following that Jeanette Müller will advise the attendees on the requirements, expectations and best practices. Before lunch the first ESRs (Early Stage Researchers) will give an update on their work progress – the other ESRs will do the same after lunch and in the afternoon. Later in the day there will be a Management Board meeting.

On the second day the Technical Review Meeting will take place. The morning and early afternoon will mainly be filled with individual reports by the ESRs and the coordinator. Later in the afternoon the fellows will be holding individual meetings with the REA and a reviewer to discuss their experience within the network.

The final day is reserved for a workshop held by baseclick GmbH. There will be more information available in the Training Programme in due time.


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